在SDS – PAGE中,配胶时需加入TEMED和过硫酸铵,它的作用是什么?但将凝胶液加入在制胶器后需在其上慢慢加入一层乙醇或异丙醇或水
In SDS – page, TEMED and ammonium persulfate need to be added when preparing glue. What is its function? However, after adding the gel solution to the glue maker, slowly add a layer of ethanol, isopropanol or water onto it
聚丙烯酰胺(PAGE)是由丙烯酰胺聚合形成的高分子 这个聚合反应是自由基聚合,需要有引发剂产生自由基将反应引发 过硫酸铵就是引发剂,而TEMED可以催化过硫酸铵产生自由基,从而加速丙烯酰胺凝胶的聚合:
Polyacrylamide (page) is a polymer formed by polymerization of acrylamide. The polymerization reaction is free radical polymerization, which requires an initiator to generate free radicals. The initiator is ammonium persulfate. TEMED can catalyze ammonium persulfate to generate free radicals, thus accelerating the polymerization of acrylamide gel:
过硫酸钠是一种无机化合物,化学式为Na2S2O8。它是过二硫酸的钠盐,是一种强氧化剂。它几乎不潮解,可以长时间储存。过硫酸钠可以通过硫酸氢钠的电解氧化制得:2 NaHSO4 → Na2S2O8 + H2氧化在铂阳极发生。过硫酸钠的标准氧化还原电位为2.1 V,高于过氧化氢(1.8 V),低于臭氧(2.2 V)。原位生成的硫酸根自由基(sulfate radical)的标准电极电势为2.7 V。
Sodium persulfate is an inorganic compound with the chemical formula Na2S2O8. It is the sodium salt of persulfate and a strong oxidant. It hardly deliquesces and can be stored for a long time. Sodium persulfate can be prepared by electrolytic oxidation of sodium bisulfate: 2 NaHSO4 → Na2S2O8 + H2 oxidation occurs at platinum anode. The standard redox potential of sodium persulfate is 2.1 V, higher than hydrogen peroxide (1.8 V) and lower than ozone (2.2 V). The standard electrode potential of the sulfate radical generated in situ is 2.7 v.
过硫酸铵、过硫酸钠、过硫酸钾都是过氧化物,我知道的一个用途,是用作合成以水为溶剂/介质的部分高聚物(塑料、树脂)的引发剂。
Ammonium persulfate, sodium persulfate and potassium persulfate are peroxides. One use I know is to be used as initiators for the synthesis of some polymers (plastics and resins) with water as solvent / medium.
引发剂的用量是非常少的,通常只有树脂量的1.5%左右。这些东西是分级出售的、根据纯度来分的,工业级的、化学级的、分析纯,价格是不一样的,具体出厂价格不清楚。
The amount of initiator is very small, usually only about 1.5% of the amount of resin. These things are sold at different levels according to purity, industrial grade, chemical grade and analytical grade. The prices are different, and the specific ex factory prices are not clear.
过硫酸铵、过硫酸钠、过硫酸钾都是过氧化物,我知道的一个用途,是用作合成以水为溶剂/介质的部分高聚物(塑料、树脂)的引发剂。引发剂的用量是非常少的,通常只有树脂量的1.5%左右。
Ammonium persulfate, sodium persulfate and potassium persulfate are peroxides. One use I know is to be used as initiators for the synthesis of some polymers (plastics and resins) with water as solvent / medium. The amount of initiator is very small, usually only about 1.5% of the amount of resin.
这些东西是分级出售的、根据纯度来分的,工业级的、化学级的、分析纯,价格是不一样的,具体出厂价格不清楚。
These things are sold at different levels according to purity, industrial grade, chemical grade and analytical grade. The prices are different, and the specific ex factory prices are not clear.
储存:过硫酸铵,但由于能释放氧而有助燃作用,储存时,必须存放在干燥、密闭的容器中,避免阳光直射、勿近热源。不可同有机物和铁锈、少量金属等还原性物质接触,以防引起过硫酸铵的分解、爆炸。
Storage: ammonium persulfate, which can release oxygen and has combustion supporting effect, must be stored in a dry and closed container to avoid direct sunlight and close to the heat source. Do not contact with organic matter, rust, a small amount of metal and other reducing substances to prevent the decomposition and explosion of ammonium persulfate.
由于潮湿的过硫酸铵粉末及其水溶液有漂白和轻微的腐蚀作用,因此使用过程中因避免眼睛、皮肤和衣物直接与其接触。
Because the wet ammonium persulfate powder and its aqueous solution have bleaching and slight corrosion, it is necessary to avoid direct contact with eyes, skin and clothes during use.
用途:
Purpose:
聚合:乳胶或丙烯酸单体聚合液、醋酸乙燃、氯乙烯等产品的引发剂,同时也是苯乙烯丙烯腈、丁二烯等胶体发生共聚作用的引发剂。
Polymerization: it is the initiator of latex or acrylic monomer polymerization solution, ethyl acetate, vinyl chloride and other products. It is also the initiator of copolymerization of styrene, acrylonitrile, butadiene and other colloids.
金属处理:金属表面的处理(例如在半导体制造业:印刷电路的清洁和蚀刻)。铜和铝表面的活化。
Metal treatment: treatment of metal surfaces (e.g. in semiconductor manufacturing: cleaning and etching of printed circuits). Activation of copper and aluminum surfaces.
化妆品:漂白配方的主要成分。
Cosmetics: the main component of bleaching formula.
纺织品:脱浆剂和漂白剂——特别为低温漂白。
Textiles: desizing agent and bleaching agent – especially low temperature bleaching.
其他:化学合成:水处理(净化);消毒剂;废气处理,有害物质氧化降解(例如汞);纸张(淀粉改性,为低温漂白特殊再制浆)。
Others: chemical synthesis: water treatment (purification); disinfectant; Waste gas treatment, oxidative degradation of hazardous substances (e.g. mercury); Paper (starch modified, special re pulping for low-temperature bleaching).
郑州天顺化工有限公司从事氢氧化钾,过硫酸铵已经多年,我们坚持真诚服务客户,更多的内容请关注我们的网站:https://www.tsswhg.com
Jinan Jinhao Chemical Co., Ltd. has been engaged in potassium hydroxide and ammonium persulfate for many years. We insist on sincere service to customers. For more information, please pay attention to our website: https://www.tsswhg.com